[6p-PB6-1~18] 12.7 Biomedical Engineering and Biochips

[6p-PB6-4] Response of Tissue Slice to Mechanical Stretching:Characteristic Cracking Pattern
as a Useful index on Medical Diagnosis

〇Keisuke Danno¹, Natsumi Okoso¹, Naohiko Nakamura², Kohta Iguchi², Masaya Ikegawa¹, Takahiro Kenmotsu¹, Kenichi Yosikawa¹ (1.Doshisha Univ., 2.Kyoto Univ.)

Keywords:Pathological diagnosis, Mechanical, Mechanical Stretching

Pathological diagnosis is usually performed based on morphological observation on tissue slices by use of optical microscopy. Here, we will report a new methodology to evaluate the disease state through the application of mechanical stress on tissue slice. By adapting the samples of liver of mouse in different disease states, including cancer generation, it was found that characteristic cracking pattern is generated dependent on the developmental stage of the disease. Through the quantitative analysis of the cracking pattern, it becomes possible to make the diagnosis of the disease state in a quantitative manner. As an important discovery, this novel method affords us the reliable differentiation between malignant tumor and non-alcoholic steatohepatitis (NASH). Such a difference is explained in terms of the difference of the strength of cell-cell adhesion. Possible further extension of such novel method as the diagnosis tool will be discussed.