[6p-PB6-1~18] 12.7 Biomedical Engineering and Biochips

[6p-PB6-5] Constructing stable cellular assemblies in the presence of soluble protein: Application of laser tweezering in a crowding medium

〇(M1)Takehiro Yamazaki¹, Syouto Tsuji¹, Koichiro Sadakane¹, Takahiro Kenmotsu¹, Kenichi Yoshikawa¹ (1.Doshisha Univ.)

Keywords:Cell, Optical tweezers, Crowding effect

In this study, we attempted to construct a stable 3D cellular structure using Albumin, a biological protein, as a polymer for crowding effect. Arbitrary cells were trapped by noninvasive laser in the presence of Albumin to align cultured cells. The wavelength of the laser was 1064 nm, and a near infrared laser was used. Thereafter, the cell population was transported to the physiological saline side by remote operation with a laser. In addition, since adhesion stability of the cell population after cell arrangement was measured using twin laser, it is reported together.