[7a-A503-1~12] 12.6 Nanobiotechnology

△ [7a-A503-12] A single virus digital bioassay using smartphone fluorescence microscopy

〇Yoshihiro Minagawa^1,2, Hiroshi Ueno^1,2, Yuuko Kawaguchi^1,2, Kazuhito Tabata^1,2, Hiroyuki Noji^1,2 (1.Dept.Appl.Chem, UTokyo, 2.JST ImPACT)

Keywords:single virus detection, digital bioassay, point of care testing

Point of care testing (POCT) is a test that conducted near side of patient whenever medical care is needed. Therefore, rapid and simple diagnosis is desirable features for POCT. Digitalization of fluorogenic enzymatic assays through the use of femtoliter-volume reactor arrays is an emerging approach to realizing highly quantitative and rapid bioassays. First, we achieved a single influenza virus digital assay by digitalization of fluorogenic enzyamtic assays. Then, we developed a compact fluorescence observation system employing smartphone.