[7p-A503-1~17] 12.6 Nanobiotechnology

[7p-A503-10] Dynamic Response of Human Periodontal Ligament to mechanical stimuli

Ayano Fujita¹, Masatoshi Morimatsu¹, 〇(PC)Masayoshi Nishiyama², Seigo Takashiba¹, Keiji Naruse¹ (1.Okayma Univ., 2.Kyoto Univ.)

Keywords:HIgh pressure, Mechanobiology, Periodontal ligament

Periodontal ligament (PDL), which connects the teeth to the alveolar bone, is always exposed to mechanical stress such as occlusal force at biological range (~MPa). However, the mechanism of remodeling of PDL in a molecular level is poorly understood. Here we study the effect of pressure on PDL fibroblasts. We used high hydrostatic pressure microscope to observe PDL fibroblasts under high pressure in real time. As a result, high hydrostatic pressure (> 20 MPa ) contracts PDL fibroblasts and actin stress fibers. Furthermore, contracted cells restart spreading on the surface after high pressure releasing process. Our data suggest excessive occlusal force induces the collapse of PDL and occlusal force at biological range affects homeostasis of PDL.