4:00 PM - 4:15 PM
[7p-A503-8] Investigation on the adsorption process of protein molecules onto water surface
by the analysis of laser scattering spectrum
Keywords:Ferritin, Laser scattering, Self-assembly
We have developed a new instrument which enables us to analyze in real-time surface tension, elasticity and viscosity of a water surface through the spectrum of the laser beam scattered at the water surface. The objective of this study is to measure these physical properties of the surface of aqueous solution of ferritin and to clarify the mechanism of the process of adsorption of ferritin molecules onto the water surface.
The results showed that, after the sample solution was poured into the cell, the surface tension has kept unchanged for several to tens of minutes until it started descending. On the other hand, the viscosity has raised just after the sample was poured. This implies that the ferritin molecules accumulated beneath the water surface without being denatured for a while and then slowly decreases the surface tension due to their self-collapsing nature.
The results showed that, after the sample solution was poured into the cell, the surface tension has kept unchanged for several to tens of minutes until it started descending. On the other hand, the viscosity has raised just after the sample was poured. This implies that the ferritin molecules accumulated beneath the water surface without being denatured for a while and then slowly decreases the surface tension due to their self-collapsing nature.