[8a-A503-1~11] 12.6 Nanobiotechnology

[8a-A503-4] Quantitative measurements of cell adhesion strengths between a macrophage and cancer cells based on single cell manipulation using cup-attached AFM chip

〇Hyonchol Kim^1,2, Kenta Ishibashi², Ayana Yamagishi¹, Miku Imaizumi², Kosuke Matsuo³, Atsushi Kira³, Yui Onomura¹, Tomoko Okada¹, Chikashi Nakamura^1,2 (1.Biomed. Res. Inst., AIST, 2.Grad. Sch. Eng., Tokyo Univ. Agric. Technol., 3.Japan Aviation Electronics Ind. Ltd.)

Keywords:AFM, Cell adhesion, Micro- and Nano-particles

Intercellular adhesion between a macrophage and cancer cells was quantitatively measured using atomic force microscopy (AFM). A cup-shaped metal hemisphere was attached to the apex of the AFM cantilever. The AFM chip approached a macrophage cell, picked up, and intercellular adhesion between the macrophage and cancer cells was quantitatively measured. In results, adhesion strength between a macrophage and highly malignant cancer cell was almost 2-fold larger than other cell pairs. These results are consistent with clinical investigations in tumor microenvironment.