[14a-F204-1~12] 12.7 Biomedical Engineering and Biochips

[14a-F204-12] Development of the PCR under the low ion PCR condition

〇Fumie Takei^1,3, Misaki Akiyama³, Kazuyuki Nobusawa², Norhayati Binti Sabani³, Huanwen Han², Kazuhiko Nakatani³, Ichiro Yamashita² (1.NDMC, 2.Graduate School of Engineering, Osaka Univ., 3.ISIR, Osaka Univ.)

Keywords:polymerase chain reaction, SNPs

A single nucleotide polymorphism (SNP) is one base differ of a gene, and it cause of each individual difference. Allele-specific Polymerase Chine Reaction (PCR) method is used to determine the SNP by the presence or absence of gene amplification. We investigated in here the SNP typing using PCR under low concetration of total ion (up to 15 mM).Development of the PCR under the low ion PCR condition.