[14p-311-1~19] 12.2 Characterization and Materials Physics

[14p-311-15] High Sensitive Polypyrrole Biosensors (Hydrogen Peroxide Sensor)

〇Mitsuyoshi Onoda¹, D.M.G. Preethichandra² (1.Univ. Hyogo, 2.C.Q.Univ.)

Keywords:rectangular pulsed electropolymerization, enzyme immobilization, nanostructured biosensor

We have discussed about a novel technique of developing a nanostructured polypyrrole biosensor for measurement of very low concentrations of hydrogen peroxide in liquid media. The proposed fabrication method is very effective in growing a nanostructured conductive polymer layer on a planar conductive substrate. In addition enzyme loading was done under a high electric field of 1 kV/m. The developed sensor provides a linear range of 0-200 mmol/l of hydrogen peroxide and a measurement sensitivity of 6.5 Acm^-2(mol/l)^-1.