[14p-F204-1~15] 12.7 Biomedical Engineering and Biochips

▼ [14p-F204-15] Fabrication of a Prototype Dual Filter CMOS Image Sensor for FRET Imaging

〇(D)Wan Shen Hee¹, Makito Haruta¹, Toshihiko Noda¹, Kiyotaka Sasagawa¹, Takashi Tokuda¹, Jun Ohta¹ (1.NAIST)

Keywords:CMOS sensor, Dual filter, Fluorescent

Förster resonance energy transfer (FRET) is the radiationless energy transfer between molecules which lies in close proximity, which is useful to investigate biological events in molecular level, for example the development of cancer. Recent trend of research had been switching towards producing image sensor capable of observing FRET phenomenon. In this work, a prototype image sensor is designed and developed in such a way that it could detect both cyan and green fluorescent light at the same time. The image sensor is equipped with two layers of color filter to allow it to detect two different fluorescent light. A blue color filter is patterned using laser after it was coated directly on the image sensor to create alternating column of photodiode which are covered by the blue filter and vice versa. Then, one side of the yellow filter is glued onto a fiber optic plate and the other side to the imaging side of the image sensor using epoxy. In this way, two diff erent photodiode columns configuration can be obtained and hence the detection of green and cyan fluorescent light on the same device is possible. Results from the sensitivity measurement of the prototype sensor shows that this dual filter design able to detect high intensity of cyan and green color light. The in vitro experiment carried out using the prototype sensor shows that this sensor capable of capturing green and cyan fluorescent light emitted by the cultured cell after excited by external light source.