[15p-413-1~8] Recent optical bio-sensing in a microscopic region

[15p-413-6] Full Field Optical Coherence Microscopy using Short Multimode Fiber Probe
and its Applications to Biological Tissues

〇Manabu Sato¹, Nishidate Izumi² (1.Yamagata Univ., 2.Tokyo Univ. of Agriculture and Technology)

Keywords:Optical coherence microscopy, Fiber optics imaging, Medical optics

We demonstrated full field optical coherence microscopy (FFOCM) using an ultrathin forward-imaging short multimode fiber (SMMF) probe of 50 μm core diameter, 125 μm diameter, and 7.4 mm length, which is a typical graded-index multimode fiber for optical communications. The axial resolution was measured to be 2.14μm, which is close to the calculated axial resolution of 2.06 μm. The lateral resolution was evaluated to be 4.38 μm using a test pattern. The contrast of the OCM images was 6.1 times higher than that of the signal images due to the coherence gate. By contacting the SMMF to the primary somatosensory cortex and the agranular insular cortex of an ex vivo rat brain, 3D OCM images of the brain were measured and internal structure information was obtained. The feasibility of an SMMF as an ultrathin forward-imaging probe in FFOCM has been demonstrated.