[15p-B6-1~16] 17.2 Graphene

△ [15p-B6-4] Study on electrochemical characteristics of enzyme/graphene electrodes for biofuel cell applications

〇(M2)Noritoshi Nakagawa¹, Ryota Takada¹, Yoshitaka Momiyama¹, Masahiro Hirano¹, Kouhei Yanai¹, Shinji Koh¹ (1.Aogaku Univ.)

Keywords:graphene, enzyme, fuel cell

We evaluated electrochemical characteristics of enzyme/graphene electrodes for enzyme-modified biofuel cell applications. Monolayer graphene sheets were grown by chemical vapor deposition (CVD) on Cu foils. To evaluate the electrochemical properties, the graphene sheets were transferred onto inert SiO₂/Si substrates by using a PMMA method. We immobilized an enzyme, fructose dehydrogenase (FDH) by using 1-pyrenebutanoicacid succinimidyl ester (PBSE) as a chemical linker on the graphene sheets. FDH catalyzes the oxidation reaction of fructose and current due to the oxidation reaction is generated by the addition of fructose. A catalytic oxidation current of up to 14.0 μA / cm² was clearly observed in the cyclic voltammetry. The FDH/graphene electrodes exhibited an electrode reaction of direct electron transfer on anode electrodes. A maximum power density of 1.4 μW/cm² was obtained in an enzyme-modified biofuel cell using an FDH/graphene electrode for the anode and a Pt electrode for the cathode.