[16a-313-1~13] 8.6 Plasma life sciences

[16a-313-4] Comparison of Cell Membrane Permeabilization between Different Types of Gas-Liquid Interfacial Plasmas

〇Toshiro Kaneko¹, Shota Sasaki¹, Yutaro Hokari¹, Yuexing Zheng¹, Keisuke Takashima¹, Makoto Kanzaki² (1.Dept. of Electronic Eng., Tohoku Univ., 2.Dept. of Biomedical Eng., Tohoku Univ.)

Keywords:gas-liquid interfacial plasma, cell membrane permeability, reactive species

In an attempt to identify the dominant factor(s) in plasma-induced cell membrane permeabilization toward developing highly-efficient and minimally-invasive transfection, the permeabilizing effects of the saline treated with different types of gas-liquid interfacial plasmas (gas-phase and in-liquid plasmas) are measured. The results show that the both plasma-treated saline can enhance the cell membrane permeability, but the efficiency in the case of the in-liquid plasma is 5 times larger than that in the gas-phase plasma. We discuss about this difference in terms of the reactive species generated in the plasma irradiated solution.