[16a-P7-1~13] English session: Joint Symposium on Nanobiotechnology and Biosensing

▲ [16a-P7-10] Peptide aptamer-based single-walled carbon nanotube field-effect transistor biosensor for cathepsin E biomarker detection

〇(D)Tung Thanh Nguyen¹, Tue Trong Phan¹, Lien Thi Ngoc Truong², Ohno Yasuhide³, Maehashi Kenzo⁴, Matsumoto Kazuhiko⁵, Biyani Manish¹, Takamura Yuzuru¹ (1.JAIST, 2.HUST, 3.Tokushima Univ., 4.Tokyo Univ.Agr.Tech., 5.Osaka Univ. ISIR)

Keywords:single-walled carbon nanotube field-effect transistor, cathepsin E, pancreatic cancer

Recently, cathepsin E (CatE) was found as promising biomarker for pancreatic cancer. Peptide aptamer which is specific to CatE has also been developed. So far, there is no report on combination of peptide aptamer and single-walled carbon nanotube field-effect transistor (SWCNT FET) as probe molecule and transducer, respectively, for CE detection. In our previous work, the activity of peptide aptamer probe molecule was confirmed for the development of SWCNT-FET aptasensor. In this work, SWCNT FET-based biosensor was further applied for early pancreatic cancer diagnostics. The minimum detectable concentration of 2.33 pM was lower than previous works. This obtained highly sensitive detection of CatE could be useful for early pancreatic cancer diagnostics and treatment.