[16a-P7-1~13] ナノバイオテクノロジーとバイオセンシングに関するジョイントシンポジウム

▲ [16a-P7-13] Transmission Surface Plasmon Resonance Imaging of Gold Grating and Silver Nanoparticle for Creatinine Detection

〇(D)Pothipor Chammari^1,2、Ounnunkad Kontad²、Lertvachirapaiboon Chutiparn¹、Shinbo Kazunari¹、Kato Keizo¹、Kaneko Futao¹、Baba Akira¹ (1.Niigata Univ. for Niigata University、2.Chiang Mai Univ. for Chiang Mai university)

キーワード：transmission surface plasmon resonance-imaging, Creatinine

The development of a simple platform for sensor applications with the good sensitivity, high selectivity, and high stability is of key attention for diagnostics and pharmaceutical analyses. In this report, we demonstrate transmission surface plasmon resonance-imaging (TSPR-i) with a microfluidic 5-channel cell as shown in Fig. 1A. Silver nanoparticles (AgNPs) were deposited with poly (diallydimethylammonium chloride) (PDADMAC) and poly (sodium 4-styrenesulfonate) (PSS) using layer-by-layer (LbL) method on gold thin film grating/substrate surfaces ^[1]. TSPR-i technique was used to study the effect of localized surface plasmon resonance (LSPR) from plasmonic AgNPs on the gold-grating/dielectric interface for the signal enhancement of creatinine detection. After the deposition of AgNPs on the gold grating substrate, a decrease of TSPR-i intensity was observed (Fig. 1B). 10 mM creatinine solution was injected into the 5-channel microfluidic cell for 1 h. As shown in Fig. 1B, the brighter TSPR image (green line) was obtained after the injection of creatinine on the AgNPs/gold grating system (Fig. 1C). In addition, 5-bilayer of AgNPs/PDADMAC film onto 10 layer of PDADMAC/PSS intermediate layers on BK7 glass slides before and after injection of creatinine was studied by UV–vis absorption spectroscopy. As shown in Figure 1D, the AgNPs/PDADMAC film was deposited onto the surface of a PDADMAC/PSS ultrathin film with the peak wavelength of 420 nm. After injection of 10 mM creatinine, the plasmon absorption band of AgNPs at 420 nm decreased.