[16p-F205-1~18] 12.7 Biomedical Engineering and Biochips

[16p-F205-13] Fluorescence detection of the labeled cells using a filter-free fluorescence sensor

〇(DC)Yongjoon Choi¹, Youichi Shinozaki², Kazuhiro Takahashi¹, Tatsuya Iwata¹, Schuichi Koizumi², Kazuaki Sawada¹ (1.Toyohashi Univ. of Tech., 2.Univ. Yamanashi.)

Keywords:filter-free, fluorescence sensor

We proposed a cell analysis system without optical filters using a filter-free fluorescence sensor which used wavelength dependent absorption depth in a Si substrate. Excitation light and fluorescence from fluorescently labeled cells were separated and the light intensities were measured at the same time by applying the proposed parameter. This result shows the filter-free fluorescence sensor can be used to detect labeled cells with various fluorescence reagents. The measured fluorescence of cells was good agreement with fluorescence intensity observed by conventional fluorescence microscopy.