[17p-413-1~10] 3.4 Biomedical optics

[17p-413-10] Analysis of triplet/dark state dynamics of fluorescent molecules in the photobleaching process

〇Nodoka Sakata¹, Satoshi Maesako¹, Kamiyama Naoto¹, Iwata Norihiro¹, Toda Keisuke¹, Suda Akira¹ (1.TUS)

Keywords:photobleaching, imaging

We determine characteristic times of excited states of fluorescent molecules, branching ratio for photobleaching, and fractional contributions of excited states to photobleaching. These results are given by analyzing the transient response of eGFP fluorescence.