[18p-144-1~12] ナノバイオ・ナノ医療に向かう技術イノベーション ～材料、デバイスから計測まで～

▲ [18p-144-10] Dual function of gold grating film in catalytic decomposition of hydrogen peroxide and its real-time monitoring transmission surface plasmon resonance signal

〇Chutiparn Lertvachirapaiboon¹、Akira Baba¹、Kazunari Shinbo¹、Keizo Kato¹ (1.Niigata Univ.)

キーワード：transmission surface plasmon resonance, silver nanoprisms, gold catalyst

Gold-coated grating substrate have been used for catalytic decomposition of hydrogen peroxide (H₂O₂) and for detection of dielectric of silver nanoprisms (AgNPrs) solution change due to oxidation of AgNPrs using transmission surface plasmon resonance (TSPR) technique. The decomposition of H₂O₂ employing gold grating film as a catalyze was 2 times faster than without gold catalyze. These were observed by changing in Dintensity of TSPP peak at the wavelength of 745 nm as a function of time. Since this system was very sensitive to H₂O₂, we further utilized this system to quantify glucose through the glucose-oxidase system. The decomposition of AgNPrs was caused by generated H₂O₂ from enzymatic oxidation of glucose using glucose oxidase enzyme. When the AgNPrs was decomposed, the increase in TSPR intensity was observed. At glucose concentration of 100 mM, the delta intensity of TSPR peak at the wavelength of 745 nm of the system with gold catalysis was greater than without gold catalysis for 4.4 times. More interestingly, at a glucose concentration of 20 mM, with gold catalysis, the delta intensity of TSPR peak was greater than without gold catalysis for 20.4 times. With gold catalysis, the significant improvement of sensitivity of glucose detection was observed. We believe that the developed technique is reliable and practical for applications in the field of biochemical analysis and clinical diagnosis.