[18p-221C-1~8] 12.7 Biomedical Engineering and Biochips

▼ [18p-221C-5] Electrospray microarray platform for bulk generation of highly monodisperse cell-like compartments

〇(DC)Kirti Sharma¹, Noritaka Minami¹, Phan Trong Tue¹, Yuzuru Takamura¹, Manish Biyani^1,2 (1.JAIST, 2.BioSeeds Corporation)

Keywords:In vitro compartmentalization (IVC), Immersed electrospray, Micro-Hole Array Electrospray system

Over the past two decades, in vitro compartmentalization (IVC) technique has been widely used to produce cell-like compartments using conventional methods of homogenizers and vortexing, microfluidic-based approaches and single-nozzle electrospray. But all these technologies have their own disadvantages leading to the search for a new technology. Here, we report to develop a novel platform by integrating immersed electrospray with micro-hole array (384 holes) to generate highly monodisperse water-in-oil or agarose-in-oil droplets of 1fL in just 5 minutes with increased library size to 10^11-12.