[19a-PA2-1~13] 3.1 Basic optics and frontier of optics

[19a-PA2-11] Three-Dimensional Image Reconstruction by Airy Beam Conversion with Bessel Beam Scanning for Two-Photon Excitation Laser Microscopy

〇Yuichi Kozawa^1,2, Sato Shunichi¹ (1.IMRAM, Tohoku Univ., 2.JST PRESTO)

Keywords:Bessel beam, Airy beam, Laser microscopy

We develop a novel method in fluorescent laser scanning microscopy that enables fast acquisition of three-dimensional images of a specimen without moving the observation plane by scanning a needle-shaped excitation spot produced by a non-diffracting Bessel beam. The generated fluorescent signal at each scanning position is converted into an Airy beam with self-bending property to extract the depth information of the specimen. Here, we demonstrate three-dimensional image reconstruction based on the principle.