[20p-PA3-1~13] 12.6 Nanobiotechnology

[20p-PA3-1] In-Liquid AFM of Calcium-dependent Morphology Change of Cadherin

〇Teiko Seki^1,2, Siew Soke Lee¹, Mitsuaki Goto¹, Eiry Kobatake², Toshihiro Akaike¹ (1.Biomat. Ctr. for Regenerative Med.Eng., FAIS, 2.School of Life Sci. and Tec., Tokyo Tech)

Keywords:AFM, Cadherin, Regenerative Medicine

In order to identify complex functions of cells, we have derived simple models for cell-cell and cell-matrix interactions to design and develop various new petri dish-fixed cell recognition biomaterials. E-cadherin-Fc chimera protein is one such biomaterial which exhibits Ca²⁺-dependent cell adhesion properties. For the purpose of investigating the correlation between its structure and function, AFM observations in liquid have been performed to reveal a morphology which was predicted from its dissociation coefficient. The results of cell culture will be also discussed.