[20p-PA4-1~23] 12.7 Biomedical Engineering and Biochips

▲ [20p-PA4-11] Automated Reverse Rotation Centrifugal Microfluidic Chip System for Reflow and Trapping of Single Cells

〇(P)Wilfred Espulgar¹, Masato Saito¹, Shohei Koyama¹, Hyota Takamatsu¹, Eiichi Tamiya¹ (1.Osaka Univ.)

Keywords:centrifugal microfluidics, single cell, small volume

Single cell trapping has proven to be one of the key strong points of microfluidics technology in the field of Life Sciences. Many reports have been presented claiming to have very high trap efficiency (~100%). However, when compared to the number of cells perfused through the trap arrays, most of the cells go to waste. This poses an issue when dealing with rare cells such as circulating tumor cells, stem cells, a cell infected by virus or parasites, or to a suspension from a biopsy sample where all cells are desired to be analyzed. To address this concern, a reflow system has been envisioned to increase the probability of particle trapping