[21p-234A-1~16] 3.4 Biomedical optics

[21p-234A-1] [Young Scientist Presentation Award Speech] High-resolution imaging by nonlinear fluorescence microscopy using repetitive stimulated transition

〇Fumihiro Dake¹, Naoki Fukutake¹, Seri Hayashi¹, Yusuke Taki¹ (1.Nikon, R&D division)

Keywords:light microscopy, fluorescence microscopy

We propose super-resolution nonlinear fluorescence microscopy with pump-probe setup that employs repetitive stimulated absorption and stimulated emission caused by intensity-modulated two-color beams. The resulting nonlinear fluorescence that undergoes such a repetitive stimulated transition is detectable as a signal via the lock-in technique. The signal is produced by the multi-ply combination of incident beams, resulting in an improvement of the three-dimensional optical resolution. Imaging results of fluorescent beads and biological samples showed that REST microscopy has superior optical resolution to conventional laser-scanning fluorescence microscopy.