[17p-A202-1~19] 12.6 Nanobiotechnology

▼ [17p-A202-9] Nanostraw membrane stamping for direct delivery of molecules into adhesive cells

〇(M2)Bowen Zhang¹, Yiming Shi¹, Miyake Takeo¹, Nakazawa Koji² (1.Waseda Univ., 2.Univ Kitakyushu)

Keywords:Au-nanostraw membrane, Cell injection

Delivering ions and molecules into the cell has become an important challenge in biological and medical research fields. However, common molecular delivery has several issues such as physical and chemical damage to functional cells. Here we present a direct delivering of molecules into NIH-3T3 cells with Au nanostraw membrane stamping that results in highly efficient delivery (> 90%) and reliability (ca. 90%). We developed Au nanostraw membrane with electroless plating on track-etched polycarbonate template and O₂ reactive ion etching (RIE). After the RIE, we confirmed Au nanostraws with a scanning electron microscope. To make a nanostraw stamp, we mounted Au nanostraws (Diameters: 400nm, 600nm, 1000nm and Lengths: 0 – 10 mm) on glass tubes with water proof glue and then pour a PBS or medium including calcine, which is an impermeable molecule into the cell membrane. Then we stamped to NIH-3T3 cell for 1 min. After incubating for 30 min, we confirmed the calcine delivery into the cells with fluorescence microscope. Furthermore, live/dead cell ratio (reliability) was confirmed by propidium iodide (PI) dye.