4:00 PM - 6:00 PM
[17p-P11-23] Leukocyte Collection and Sorting from Whole Blood using Weir Structure-baced filters
Keywords:cell sorting
This report presents the cell sorting capability of the weir structured-based filters in the designed microfluidic chip. Sorting and separation of cells from a heterogeneous mixture is a vital process in any cell-related research. The biophysical characteristics of the cell such as morphology, size, density, and dielectric susceptibility are commonly utilized in a label-free sorting method but often lacks specificity. The use of labels or biological markers is common alternative but succeeding processes can have an influence on the cellular phenotype. In this study, the cell sorting capability is associated to the deterministic cell rolling of related to the rapid formation and rapid dissociation of adhesive bond on a contact surface. The weir structure is subjected to different cells available to test its compatibility and with the ultimate goal of sorting the type of cells in a blood, specifically, white blood cells. The design has been further modified based on the results. We already have separated two type of cells. By improving the collect system, we can evaluate these cells and further improve the chip. With this result, an alternative sorting method can be realized that can be utilized for low concentration cell volume; a common downside of commercialized machines and protocols. To enhance the sorting capability, optimized coating will be needed which can interact with the different integrin present on the cell.