4:00 PM - 6:00 PM
▲ [17p-P11-28] Receptor protein analysis of single immune cells using centrifugal microfluidic device
Keywords:Microfluidic chips, single cell analysis, Real blood tests
Cell membrane receptor proteins play important roles as a mechanism for receiving cell signals. FACS is widely used to analyze them, but it cannot simultaneously detect all receptor proteins on single cell’s membrane and requires many cells. Therefore, we are developing microfluidic devices that totally analyze expressed receptor proteins in single cell level and can analyze small cell population.
In our experiments, the microfluidic devices that analyze expressed receptor proteins in single cell level from small cell population were developed. Two types of cells were distinguished by this method as trial and demonstrate the utility of the device. Agreement of the result of it and that of FACS showed the reliability of developed analysis method. During the experiments, we use the new outsoucred software to analyze the intensity of the single cell. The outsoucred software could visually show the background of the whole design and optimize the threshold of the fluorescence signal in order to decrease the background noise. Besides this, the real blood sample was also tested. We collected the monocyte cells and injected it into the chip after separation from whole blood. We could distinguish the different type of cells within the monocyte cells via immunostaining protocol which prove that our chip has the realiability to be used in single cell analysis. We believe that by using this centrifugal device, we could develop a new profiling scheme for single cell analysis.
In our experiments, the microfluidic devices that analyze expressed receptor proteins in single cell level from small cell population were developed. Two types of cells were distinguished by this method as trial and demonstrate the utility of the device. Agreement of the result of it and that of FACS showed the reliability of developed analysis method. During the experiments, we use the new outsoucred software to analyze the intensity of the single cell. The outsoucred software could visually show the background of the whole design and optimize the threshold of the fluorescence signal in order to decrease the background noise. Besides this, the real blood sample was also tested. We collected the monocyte cells and injected it into the chip after separation from whole blood. We could distinguish the different type of cells within the monocyte cells via immunostaining protocol which prove that our chip has the realiability to be used in single cell analysis. We believe that by using this centrifugal device, we could develop a new profiling scheme for single cell analysis.