[18p-F306-1~17] 12.7 Biomedical Engineering and Biochips

[18p-F306-2] Lipid derivative molecule modified interface for exosome tethering

〇(B)Akiko Iwaya¹, Hiromi Kuramochi¹, Kyohei Okubo¹, Rei Okamura¹, Takanori Ichiki^1,2 (1.The University of Tokyo, 2.iCONM)

Keywords:exosome, nanoarray, single particle snalysis

Exosomes are extracellular vesicles with a diameter of 30-100 nm. We developed tethering nanoarrays that allow individual exosomes to be immobilized. The tethering nanoarray is composed of polyethyleneglycol (PEG)-lipid molecules, at which lipid bilayer membrane of exosomes is attached via hydrophobic interaction. To reveal an ideal condition on the preparation of PEG-lipid modified surface, the performance of the tethering nanoarray was extensively studied by changing process parameters including reaction time of silane coupling agents and length of PEG-lipid molecules. Exosome suspensions that ware derived from leukemia HL60 cell lines ware flown over the nanoarrays, then the nanoarray chip was observed using atomic force microscope and we discuss about the influence of PEG-lipid lengths on immobilization of exosomes.