[20p-P2-1~13] 3.4 Biomedical optics

[20p-P2-12] Evaluation of photodamage on living cells due to irradiation of blue laser light

〇Keisuke Takahashi¹, Shohei Kawahara¹, Naohiro Kanamaru¹, Testuya Matsuyama¹, Kenji Wada¹, Ai Kawakita², Kaori Murata², Kenji Sugimoto² (1.Engineering, Osaka Pref. Univ., 2.Life and Environmental Sciences, Osaka Pref. Univ.)

Keywords:live cell imaging, blue light

To examine the effect of blue light on living cells, a quantitative evaluation of the toxicity caused by irradiating blue laser light was carried out using a live cell imaging method. We estimated irradiation energies resulting in a cell survival rate of 50 % for the irradiation of 450 nm blue laser light to the nucleus and cytoplasm of living cells. In the case of irradiation intensities of 50, 100 W/cm², the irradiation energies resulting in a cell survival rate of 50 % were about 100 kJ/cm² for both nucleus and cytoplasm irradiation, indicating the equal toxicity. For the irradiation intensities of 400, 800 W/cm², the toxicity for living cells caused by irradiation to the nucleus was confirmed to be higher than that to the cytoplasm.