[18p-E202-1~18] 12.7 Biomedical Engineering and Biochips

▲ [18p-E202-1] [Young Scientist Presentation Award Speech] Development of laser-cut microfluidic paper-based analytical device with sucrose valve for automated competitive ELISA of Aflatoxin B₁

〇Charernchai Sumamal¹, Miyuki Chikae¹, Wanida Wonsawat², Daisuke Hirose¹, Phan Trong Tue³, Yuzuru Takamura¹ (1.JAIST, 2.Suan Sunandha Rajabhat Univ., 3.Tokyo Tech.)

Keywords:automated paper-based device, compettive ELISA, Aflatoxin B1

In this work, we report a fully automated competitive enzyme linked immunosorbent assay (ELISA) using a laser-cut paper-based device (µPAD) integrated with sucrose valve for the detection of a small-sized target, aflatoxin B₁ (AFB₁), which is a carcinogenic agent.

In previous work, our group fabricated µPAD using inkjet printing (Apilux et al., 2013). However, the fabrication was complicated and time consuming. In this study, the conventional laser platform is introduced to remove the hydrophilic nitrocellulose membrane (NCM) from the cutline of the designed pattern, and thus creating a hydrophobic barrier along the edge of the cutline. An ease of use, accuracy, reproducibility and device production speed were improved (30 times faster than inkjet printing) by laser cutting.

The competitive ELISA is performed sequentially in 3-steps on a laser-cut device integrated with sucrose valve. Due to the competitive ELISA, higher sample concentration results in weaker signal. This approach indicates further potential in automating sequential delivery of reagents for competitive ELISA, and is expected to detect AFB₁ for user-friendly and on-site measurements.