[18p-E203-1~16] 12.6 Nanobiotechnology

[18p-E203-14] Assay and interface preparation for detection of Candida bacteria using the plasmonic chip

〇Seika Shimizu¹, Hiroshi Kurita², Mitsuo Umetsu³, Hikaru Nakazawa³, Keiko Tawa¹ (1.Kwansei Gakuin Univ., 2.Shinshu Univ., 3.Tohoku Univ.)

Keywords:plasmonic chip, candida bacteria, immunity

Oral Candida contained in saliva has been indicated to be a marker for measuring immunity. In the previous study, Candida mannan was quantitatively measured with plasmonic chip by grating-coupled surface plasmon-field enhanced fluorescence (GC-SPF). In this study, we developed a Candida assay to detection of Candida bacteria in human saliva samples and prepared the interface for sandwich assay on the chip. The plasmonic chip was prepared by UV nano-imprint method and silver coating by rf-sputtering. Two types of capture interfaces with Protein G and Protein A fragment were prepared. After immobilization of anti-C.albicans antibody, heat-treated human saliva sample was injected. As a result of detection with a labeled fluorescence antibody, significant differences were evaluated between saliva samples and control. Therefore, Candida bacteria in human saliva samples was measured with the plasmonic sensor chip.