[18p-E208-1~13] 4.1 Plasmonics and Nanophotonics

▲ [18p-E208-12] Selective surface-enhanced Raman scattering platform with functional phage as a bio-receptor

〇(P)Jong-Min Lee¹, Na-Na Jeong², Vasanthan Devaraj¹, Minjun Kim³, Samir Adhiari³, Won-Geun Kim¹, Jong-Ryeul Sohn², Donghan Lee³, Jin-Woo Oh¹ (1.Pusan Natl. Univ., 2.Korea Univ., 3.Chungnam Natl. Univ.)

Keywords:SERS, Bio-receptor, M13 bacteriophage

Phage display, an attracting attention, and recipient of 2018 Nobel Prize in Chemistry is a method for screening functional bacteriophages (phage) with high binding affinity for the target molecules, can enhance selectivity and sensitivity when applied to sensors. In particular, the functional phage is expected to be suitable for the SERS platform because low selectivity is a problem even though the SERS platform is known to have high sensitivity. Here we present a method for applying functional phage to the SERS platform as a bio-receptor. The binding affinity of the functional phage for the target substance was verified by the isotherm titration calorimetry (ITC). As a result, it was confirmed that the selectivity and sensitivity of the SERS platform into which the phage was introduced were enhanced in proportion to the binding affinity.