[19a-E203-1~12] 12.6 Nanobiotechnology

▲ [19a-E203-12] Adsorption and electrochemical study of cage-shape protein with GBP aptamer

〇Huanwen Han¹, Norhayati Binti Sabani², Naofumi Okamoto³, Masakazu Nakamura³, Ichiro Yamashita^1,4 (1.Grad. Sch. of Eng., Osaka Univ., 2.ISIR, Osaka Univ., 3.Div. of Mater. Sci., NAIST, 4.Sci. Res. Alli. Lab., Osaka Univ.)

Keywords:GBP aptamer, Electrochemical impedance spectroscopy (EIS), biosensor

Aptamers are the bio-material which could bind to a specific target, and attracting researchers attention with the high affinity and specificity. Some peptide aptamers can adsorb inorganic materials which have great potential to connect the organic molecules and inorganic materials. In our previous work, a small cage-shaped protein was genetically modified to have two kinds of peptide aptamers on its outer surface. In this study, we expand this design to realized a biosensor with EIS. We plan to modify a cage-shaped protein which has electrode binding aptamers and one target bio-marker aptamers. It is expected that immersing an electrode into such recombinant protein solution would cover the electrode surface by the protein. As a result, the sensor electrode surface displays target-specific aptamers.