[21p-E206-1~10] 3.4 Biomedical optics

▲ [21p-E206-2] Simultaneous Raman-Fluorescence Flow Cytometry

〇(D)Matthew Lindley¹, Kotaro Hiramatsu^1,2, Fukashi Shibata³, Tsuyoshi Takeshita³, Shigeyuki Kawano¹, Keisuke Goda^1,4,5 (1.UTokyo, 2.JSTA, 3.Algal Bio Co. Ltd., 4.UCLA, 5.Wuhan Univ.)

Keywords:Raman, fluorescence, flow Cytometry

We demonstrate simultaneous Raman-fluorescence flow cytometry. The flow cytometer consists of a femtosecond Ti:sapphire laser coupled with a homemade pulse pair generator and microfluidic chip. The pulse pair generator rapidly scans optical delay to accomplish interferometer-based Fourier-transform coherent anti-Stokes Raman scattering and Fourier-transform two-photon excitation measurements. Sample cells are suspended in buffer solution and pumped through the microfluidic chip, where acoustic focusing is used to pass them singly through the measurement position. Using this scheme, we measured metabolic response of the microalga Haematococcus pluvialis to four different photo-stress conditions using Raman scattering from its secondary metabolite astaxanthin and autofluorescence from its chlorophyll and chlorophyll catabolites at a throughput of 10 events/s.