[10p-PA2-1~16] 12.6 Nanobiotechnology

△ [10p-PA2-12] Enzyme Immobilization in Glass Fiber Filters for Enzymatic Cascade Reactions in a Flow-Through System

〇Kai Sasaki^1,2, Yoshimoto Makoto^1,3, Nicolas Gheczy¹, Peter Walde¹ (1.Dept. of Materials, ETH Zurich, 2.Grad. School of Organic Materials Science, Yamagata Univ., 3.Dept. of Applied Chemistry, Yamaguchi Univ.)

Keywords:Carbonic anhydrase, Enzymatic cascade reaction, Flow-through reactor

To control CO₂concentration in the atmosphere under mild conditions, various bioreactors were developed by using carbonic anhydrase (CA), which is known to catalyze the hydration of CO₂to yield HCO₃⁻(aq) and H⁺(aq) in living systems. We have investigated a model cascade reaction using bovine CA (BCA) and horseradish peroxidase (HRP) for basic studies in a glass fiber filter-based flow-through reactor. To immobilize BCA and HRP on the filters, dendronized polymer-enzyme conjugates were first prepared which then were adsorbed in the filter and finally examined with a BCA-HRP-catalyzed cascade reaction.