[10p-PA3-1~21] 12.7 Biomedical Engineering and Biochips

[10p-PA3-14] Exploration of Regeneration Technique for Myoglobin Biosensor using Interdigitated Electrodes

〇Shiyo Fujishiro¹, Hitoshi Ohnuki¹, Daiki Tsuya², Haiyun Wu¹, Hideaki Endo¹ (1.Tokyo Univ. of Marine Sci. & Tech., 2.NIMS)

Keywords:biosensor, impedance, antigen-antibody interaction

We have developed a myoglobin biosensor which shows high response and high selectivity. But this sensor cannot be reused after the first measurement. Thus the problem of this sensor is the production cost and the preparation time. To solve this problem we developed a regeneratable sensor using Protein G’ which binds strongly with IgG-type antibody. Protein G’ is known to bind strongly with IgG-type antibody under neutral environment while it cuts the bind under acidic one. We employed this function to build the regeneratable sensor.