[9a-W641-1~12] 3.4 Biomedical optics

[9a-W641-12] Label free imaging of mitochondria dynamics with photothermal microscopy

〇Jun Miyazaki¹, Yasunobu Toumon¹ (1.Wakayama Univ.)

Keywords:photothermal microscopy, Label-free imaging

Various organelles inside live cells was observed without use of any label by means of a highly sensitive photothermal microscopy. Simultaneous dual-wavelength photothermal imaging allows us to identify mitochondria and related organelles which are presumably attributed to autophagosomes or autolysosomes. Real-time and time-lapse imaging revealed morphological dynamics of mitochondria and related organelles under physiological treatments. Morphological change of mitochondria from filamentous to round structure was clearly observed after membrane potential depolarization induced by chemical inhibitor. In contrast to fluorescence imaging, this method can observe the dynamics of various organelles, especially mitochondria without labeling, Therefore, it would be useful to understand their essential function in biomedical sciences and has a potential for an efficient diagnostic tool of mitochondria related deceases.