[9p-W241-1~12] 8.4 Plasma life sciences

[9p-W241-3] Elucidation of molecules in plasma-activated lactate for selective killing cancer cells

Yugo Hosoi¹, Shogo Maeda¹, 〇Kenji Ishikawa¹, Jun Yoshitake², Takahiro Shibata², Hiromasa Tanaka¹, Hiroshi Hashizume¹, Fumitaka Kikkawa³, Masaaki Mizuno³, Masaru Hori¹ (1.Nagoya Univ., 2.Nagoya Univ. Agri., 3.Nagoya Univ. Med.)

Keywords:Plasma activated lactate, Antitumor effect

Selective killing of cancer cells incubated in non-equilibrium atmospheric pressure plasma (NEAPP)-activated medium (PAM) has been reported. Base on the intracellular response mechanism of antitumor effects, reactive oxygen and nitrogen species (RONS) in the PAM induced intracellular oxidative stress and lead apoptotic cell death. Thus, the PAM acts as a cellular stressor to rapidly induce fluid-phase endocytosis, lysosome biogenesis and autophagy. Recently, selective killing effect was also reported on the incubation in plasma-activated lactated Ringer's solution (Lactec), so called as PAL. However, the cancer cells incubated in the PAL received less oxidative stress than that of the PAM. Therefore, we have focused on authophagy as another mechanism for the PAL-induced cell-death.