[10p-Z12-1~21] 12.6 Nanobiotechnology

[10p-Z12-1] Detection of cell surface molecules with acquisition of cells into cup-shaped microelectrodes and electrochemiluminescence measurement

Airi Kuriyama¹, Kohki Uchiyama^1,2, Tomoyuki Kamata³, Dai Kato³, Naoshi Kojima³, Shohei Yamamura³, 〇Hyonchol Kim^1,2,4 (1.Fac.. Eng., Tokyo Univ. Agric. Technol., 2.Cell. Mol. Biotechnol. Res. Inst., AIST, 3.Health Med. Res. Inst., AIST, 4.Grad. Sch. Eng., Tokyo Univ. Agric. Technol)

Keywords:Electrochemiluminescence, Cancer cell, Micro and nano-particles

Electrochemiluminescence (ECL)-based detection was applied for the identification of cell surface marker molecules. Cup-shaped microelectode, which has almost same size with that of cell, was fabricated. Cancer cells were reacted with ECL probe-conjugated anti-EpCAM antibodies, and were captured to inner concaves of the cups. After the application of electric voltage, EpCAM molecules were successfully detected by ECL irradiation from the cup electrodes.