[10p-Z28-1~21] 3.4 Biomedical optics

[10p-Z28-13] Irradiation site dependence of phototoxicity by blue laser irradiation to living cells

〇Mikiya Yamaguchi¹, Noboru Osaka¹, Tetsuya Matsuyama¹, Kenji Wada¹, Koichi Okamoto¹, Ai Kawakita², Kaori Murata², Kenji Sugimoto² (1.Engineering Osaka Pref.Univ., 2.Life and Environmental Sciences Osaka Pref.Univ.)

Keywords:blue light, phototoxicity, PCNA

In live cell imaging, we have shown that by observing the dynamics of proliferating cell nuclear antigen (PCNA) after irradiation with blue laser light, blue light causes DNA damage in living cells. Irradiation site dependence of DNA damage was examined by irradiating the cell nucleus and cytoplasm of cells in the cell cycle with laser light. After laser light irradiation, PCNA accumulation was observed in cell nucleus irradiation, but accumulation in cytoplasm irradiation was not seen.