The 81st JSAP Autumn Meeting, 2020

Presentation information

Oral presentation

3 Optics and Photonics » 3.4 Biomedical optics

[10p-Z28-1~21] 3.4 Biomedical optics

Thu. Sep 10, 2020 1:30 PM - 7:30 PM Z28

Izumi Nishidate(TUAT), Katsumasa Fujita(Osaka Univ.), Yuji Matsuura(Tohoku Univ.), Yasuyuki Tsunoi(防衛医大)

3:00 PM - 3:15 PM

[10p-Z28-6] Fluorescence Lifetime Measurement with High Temporal Resolution through Stimulated Emission

〇(D)khalil Ur Rehman1, Subir Das1, Fu-Jen Kao1 (1.Nat Yang-Ming Univ)

Keywords:Temporal Resolution, Fluorescence Lifetime, Stimulation Emission

Stimulation emission (SE) is a versatile nonlinear optical technique that exhibits the novelties of sub-diffraction imaging, detection of “dark” (non-fluorescent) fluorophores, fluorescence imaging at an extended working distance [1-3]
In this work, we have demonstrated fluorescence lifetime measurement through stimulation emission (SE) in pump-probe configuration with very high temporal resolution (~4 ps), compared with the temporal resolution (~ 30 ps) of the conventional time-correlated single-photon counting (TCSPC).
Experimentally, the temporal resolution of TCSPC is limited by the electronic jittering in the photon counting detector and the pulsed light source [4]. In the pump-probe measurements, an auto-correlator is configured to control the time delay with high precision, which is only limited by the temporal response (jittering) of the synchronizing photodiode. In this way, a temporal resolution of approximately 4 picoseconds is achieved. The technique is well suited for investigating very short lifetime fluorophores. Additionally, its implementation is cost-effective and robust. ATTO647N is used throughout the demonstration.