[11a-Z12-1~10] 12.6 Nanobiotechnology

[11a-Z12-6] Evaluation of plasma-induced micropores on cell membrane for effective gene transfection

〇Tatsuya Kitazaki¹, Shinya Kumagai¹ (1.Meijo Univ.)

Keywords:gene transfection

Non-thermal atmospheric pressure plasma (NTAPP) has been actively studied for biomedical applications. One of the promising applications is gene transfer. In the application, cells are irradiated with NTAPP for effective gene transfection. However, detailed mechanism of gene transfection using NTAPP was not clear. In this study, we irradiated cells with NTAPP to introduce two fluorescent reagents. We evaluated each introduction rate to analyze the sizes of plasma-induced micropores on the cell membrane which were used for substance transfer into cells. We also analyzed how long the micropores existed on the cell membrane. Each introduction rate was almost 100% in the early stage after plasma-irradiation. As time elapsed to several tens of hours, the rate decreased to 0%. We found each introduction rate had different decreasing tendency. The difference indicated that the introduction to cells can be affected by three-dimensional structures of fluorescent molecules.