[9a-Z12-1~11] 12.7 Biomedical Engineering and Biochips

△ [9a-Z12-1] Evaluation of epithelial permeation of amphipathic phospholipid polymers by pH perturbation assay

〇(DC)Hiroaki Hatano¹, Akira Matsumoto^1,2, Yuji Miyahara^1,2, Tatsuro Goda³ (1.Grad. Sch. Life Sci. Eng., TMDU, 2.Inst. Biomater. Bioeng., TMDU, 3.Toyo Univ.)

Keywords:amphipathic phospholipid polymer, pH perturbation assay, tight junction

The epithelial barrier, which composed of epithelial cells with tight junction, maintains homeostasis. On the other hand, it blocks drug absorption into the human body. Therefore, there is a high demand for carrier molecules that can deliver the biomolecule/drugs through the epithelial barrier in drug delivery systems. The energy-independent permeability of a phospholipid-mimicked amphiphilic random copolymer composed of 2-methacryloyloxyethyl phosphorylcholine (MPC) and n-butyl methacrylate (BMA) through cell membrane was discovered. Therefore, it can be expected as a carrier molecule. In this study, we evaluated its usefulness of phospholipid-mimicked amphiphilic random copolymer as an epithelial carrier by the ISFET based pH perturbation assay, which enables highly sensitive and selective evaluation of ion barrier cell membranes/tight junctions.