[9p-Z17-1~22] 3.1 Basic optics and frontier of optics

▲ [9p-Z17-21] Optical Trapping and Assembling of Protein at Solution Surface

〇(D)Po-Wei YI^1,2, Tetsuhiro Kudo¹, Eri Chatani³, Ryohei Yasukuni², Yoichiroh Hosokawa², Teruki Sugiyama^1,2, Hiroshi Masuhara¹ (1.National Chiao-Tung Univ., 2.Nara Inst. Sci. Tech., 3.Kobe Univ.)

Keywords:Optical Trapping, Solution Surface, Optically Evolved Assembling

Optical trapping has been utilized widely in biology and physics for manipulating nano- and microscale objects such as molecules and particles. We have been studying molecular crystallization and polystyrene (PS) nanoparticles (NPs) assembling at solution surface and glass/solution interface, where molecular clusters and NPs are trapped at the focus, extending to the outside and reaching a few tens µm. This phenomenon “Optically Evolved Assembling” was also demonstrated for a highly concentrated solution of lysozyme protein, and the large expansion of their assembly was confirmed by transmission imaging. In this work, we study the phenomenon systematically by applying fluorescence imaging and Raman micro-spectroscopy.