[12a-A407-1~11] 12.6 Nanobiotechnology

▼ [12a-A407-5] Organic Solvent-Mediator for Controlling Self-Assembly of Peptide Nanowires on 2D Materials

〇(D)ROBERT JOSE CCORAHUA¹, Hironaga Noguchi¹, Kazunori Motai¹, Yuhei Hayamizu¹ (1.Tokyo Institute of Technology)

Keywords:Self-assembly, Peptide, 2D materials

The controllable nanoscale self-assembly of biomolecules, such as peptides, on 2D materials is an important issue in establishing functional bio/solid interfaces for biosensors. So far, we established method to control the self-assembly by applying electrochemical bias¹ and designing new sequence of peptides². Herein, a methanol (MeOH)-mediated self-assembly of Y(GA)₄Y peptide (Y4Y) was undergone at concentrations ranging from 0% to 50% of MeOH, showing that the threshold of self-assembly is 25% on graphene and 20% on MoS₂, with a Y4Y coverage of ~50% on both. Higher MeOH concentrations resulted in no assembly, thus evidencing the effect of MeOH to control the Y4Y structure on 2D materials. Additionally, we showed that after an initial water-mediated incubation, the assembly is destroyed in a MeOH-mediated post-incubation (PI). However, in a 10%MeOH-mediated initial incubation, the assembly remains stable to up 20%MeOH-mediated PI, indicating a distinct formed self-assembled structure.