[12p-PA6-1~15] 12.6 Nanobiotechnology

[12p-PA6-1] Preparation of Oriented Molecularly Imprinted Sensing Cavities for Tumor Marker Glycoprotein

Takahiro Morishige¹, Hiroki Matsumoto¹, 〇Eri Takano¹, Hirobumi Sunayama¹, Yukiya Kitayama¹, Toshifumi Takeuchi¹ (1.Kobe Univ.)

Keywords:molecular imprinting, glycoproteins, tumor markers

In this study, we prepared oriented molecularly imprinted sensing cavities for tumor marker glycoproteins. A boronic acid, capable of forming a reversible covalent bond with sugar moieties, was placed in the imprinted cavities for the multiple recognition of target proteins with high sensitivity and high selectivity. An apparent dissociation constant was estimated to be 10⁸ to 10⁹ M (SPR) or 10¹⁰ M (fluorescent) from the obtained binding isotherms, confirming that the sensitive detection of tumor marker glycoproteins can be achieved by the present molecular imprinted nanocavities bearing orthogonal interaction sites.