[12p-PA6-1~15] 12.6 Nanobiotechnology

[12p-PA6-13] Development of Spatiotemporally Controlled Real-time Crystallization Method for Protein Molecules

Yuka Nakasuji¹, 〇Atsushi Miura^1,2, Sho Fujii^1,2, Kosei Ueno^1,2 (1.Hokkaido Univ., 2.Fac. Sci., Hokkaido Univ.)

Keywords:Laser trapping, Protein, Real-time crystallization

Optically-induced crystallization of biomolecules has been attracting much attention. A laser trapping is a potential approach to induce in situ and real-time crystallization of small biomolecules such as amino acids with spatiotempoal controllabilities. Meanwhile, in situ and real-time crystallization of protein molecules has not been hitherto achieved. Crystallization of substances is in general an issue of the concentration (i.e. supersaturation), molecular orientation and ordering of the molecule. To realize laser trapping-induced in situ and real-time crystallization of proteins, we should understand how protein concentration and ordering/orientation change under photon pressure of a trapping laser. In this presentation, we investigated the origin of the retardation to realize in situ and real-time laser-trapping-induced crystallization of protein molecules by means of Raman and polarized Raman (p-Raman) microspectroscopies.