[13a-A408-1~12] 12.7 Biomedical Engineering and Biochips

[13a-A408-3] Development of microfluidic multiplexer for multiplexed single cells analysis using normally-closed microvalves

〇Shunsuke Hagihara¹, Shunya Okamoto¹, Hidetaka Uno², Zhi Hong Wang², Tsuneo Urisu², Shinsuke Ishigaki³, Yuzuru Takamura⁴, Yoshiaki Ukita¹ (1.Univ. Yamanashi, 2.Nagoya Univ. IIFS, 3.Nagoya Univ. Sch. Med, 4.JAIST)

Keywords:normally-closed microvalves, Diaphragm structure, multiplexer

The culture type planar patch clamp technology is a technology capable of realizing a multipoint ion channel current of a plurality of cells. If a mechanism (multiplexer) for selecting one cell as a target from a plurality of cells and connecting a flow path is realized, not only this advancement but also development to single cell gene extraction and the like can be performed. In this presentation, we report that the construction of a multiplexer using a normally closed (NC) microvalve and its effectiveness for selective multipoint measurement have been demonstrated.