[13p-A407-1~16] 12.6 Nanobiotechnology

[13p-A407-11] Detection of molecules expressed on cell surface based on the electrochemiluminescence measurement by using cup-shaped microelectrodes

Airi Kuriyama^1,2, Tomoyuki Kamata¹, Dai Kato¹, Naoshi Kojima¹, Shohei Yamamura³, 〇Hyonchol Kim^1,2,4 (1.Biomed. Res. Inst., AIST, 2.Fac.. Eng., Tokyo Univ. Agric. Technol., 3.Health Res. Inst., AIST, 4.Grad. Sch. Eng., Tokyo Univ. Agric. Technol.)

Keywords:Cancer cells, Electrochemiluminescence, Micro- and nano-particles

Electrochemiluminescence (ECL)-based measurement was applied for the detection of cell surface molecules to improve detection sensitivity of circulating tumor cell (CTC) diagnosis. Cup-shaped microelectode was fabricated, and cancer cells on which ruthenium (an ECL compound)-conjugated anti-EpCAM (a typical CTC marker) antibodies were reacted were trapped to inner concaves of the cups. After the application of electric voltage, expressions of EpCAMs were successfully confirmed by ECL irradiation from the cancer cells.