[13p-A407-1~16] 12.6 Nanobiotechnology

△ [13p-A407-9] Extracellular deuterium exchanged glutamate concentrations dependency of spontaneous neuronal activity

〇Wataru Minoshima^1,2, Kyoko Masui^1,2, Chie Hosokawa^1,3, Tomomi Tani⁴, Hidekazu Ishitobi^1,2, Yasushi Inouye^1,2 (1.Photobio-OIL AIST, 2.Front. Biosci. Osaka Univ., 3.Osaka City Univ., 4.Biomedical AIST)

Keywords:Cultured Neuronal Network, Spontaneous Neuronal Activity, Deuterated Glutamate

For future bioimaging applications, we analyzed the physiological effects of glutamate in the cultured neuronal network, in which hydrogen molecules were replaced with deuterium. We analyzed the spontaneous activity frequency depending on the concentration of extracellular glutamate (Glu) and deuterated glutamate (GluD) in neural networks cultivated on multielectrode arrays. The frequency of spontaneous activity increased depending on the increasement of extracellular Glu or GluD concentration. In addition, glutamate receptor-specific inhibitors suppressed glutamate concentration dependent increase in spontaneous activity. These results revealed that GluD has the same physiological effects as Glu in cultured neural network.