[14a-PB1-1~12] 3.1 Basic optics and frontier of optics

▲ [14a-PB1-4] Fabrication of Amyloid Fibrils of Cytochrome c Disulfide Dimers by Optical Trapping

〇(M2)ChunLiang Chiu¹, PeiHua Lo¹, ChiehJu Chang¹, Hongxu Yang², Shun Hirota², Hiroshi Masuhara^1,3, Teruki Sugiyama^1,2,3 (1.Department of Applied Chemistry, National Chiao Tung Univ., Taiwan, 2.Division of Materials Science, NAIST, Japan, 3.Center for Emergent Functional Matter Science, National Chiao Tung Univ., Taiwan)

Keywords:Optical trapping, Amyloids, Morphology

Our group has successfully demonstrated optical trapping-induced amyloid fibril formation of a domain-swapped dimer of cytochrome c (cyt c). Optical trapping with a focused laser beam increases the local concentration of cyt c at the laser focus, where the conformation of cyt c is deformed, achieving nucleation of amyloid fibrils. Here, we apply this optical trapping method to various disulfide dimers of cyt c mutants (G45C, T58C, A83C, and E104C) to fabricate amyloid fibrils and discuss different morphologies of their amyloid fibrils, depending on the direction of laser polarization.