[14a-PB1-1~12] 3.1 光学基礎・光学新領域

▲ [14a-PB1-4] Fabrication of Amyloid Fibrils of Cytochrome c Disulfide Dimers by Optical Trapping

〇(M2)ChunLiang Chiu¹、PeiHua Lo¹、ChiehJu Chang¹、Hongxu Yang²、Shun Hirota²、Hiroshi Masuhara^1,3、Teruki Sugiyama^1,2,3 (1.Department of Applied Chemistry, National Chiao Tung Univ., Taiwan、2.Division of Materials Science, NAIST, Japan、3.Center for Emergent Functional Matter Science, National Chiao Tung Univ., Taiwan)

キーワード：Optical trapping, Amyloids, Morphology

Our group has successfully demonstrated optical trapping-induced amyloid fibril formation of a domain-swapped dimer of cytochrome c (cyt c). Optical trapping with a focused laser beam increases the local concentration of cyt c at the laser focus, where the conformation of cyt c is deformed, achieving nucleation of amyloid fibrils. Here, we apply this optical trapping method to various disulfide dimers of cyt c mutants (G45C, T58C, A83C, and E104C) to fabricate amyloid fibrils and discuss different morphologies of their amyloid fibrils, depending on the direction of laser polarization.